Adenovirus PCR

Microbiology

Notes

 

  • Adenovirus is detected by PCR or immunofluorescence.
  • Adenovirus may cause a variety of clinical manifestations including (but not exclusively) conjunctivitis, pharyngitis, pneumonitis, haemorrhagic cystitis and gastroenteritis
  • Also, rarely and usually in immunocompromised patients, CNS infection, myocarditis, hepatitis, tubulointerstitial nephritis and disseminated disease
  • Tested following specific request

Sample requirements

 

Viral eye swab

Viral throat swab

Check swab expiry date before use

(Date is in YYMMDD format)

 

 

 

 

Nasopharyngeal aspirate (NPA)

 

 

 

 

 

Urine - 30 mL sterile universal container

 

 

 

 

 

4ml of blood taken into an EDTA Tube

 

In selected circumstances:

stool sample - stool container with spoon

CSF - 30 mL sterile universal container

(a minimum CSF volume of 200 µl is required for reliable testing)

Viral swab

 

 

Trachea suction set for NPA

 

 30mL sterile universal container

 

 4 mL EDTA tube

 

 

 

Required information

 Relevant clinical details

Storage/transport

All samples should be transported to the laboratory as quickly as possible at ambient temperature.

If transport is delayed then store at fridge temperature. Swab samples will remain viable for up to 96 hours and other samples will remain viable for up to 72 hours.

Turnaround time

Depending on sample type, either tested locally  or referred to the Regional Reference Centre

Up to 10 days


Page last reviewed: 12/09/2017