Neisseria gonorrhoeae (GC) PCR

Microbiology

Notes

Chlamydia samples on the PCR preparator

  • Neisseria gonorrhoeae (also known as Gonococcus or GC) can be detected by PCR at the same time as Chlamydia trachomatis testing.
  • The prevalence of gonorrhoea in the population is very low, so even with a highly sensitive and specific test there is a risk of false-positive results. Therefore, all positive results will need to be confirmed by a repeat sample and culture where possible. Requests should be made only on symptomatic patients, contacts or those with other risk factors.
  • The test is not validated for rectal and pharyngeal samples and the possibility of false positive and negative results may be higher with these sample types
  • Visibly blood stained samples and heavily purulent samples are not desirable as these may lead to false negative results
  • Urine samples from patients using Replens vaginal moisturiser may give invalid or false negative results
  • Urine samples from female patients are relatively insensitive and are not recommended
  • See also:

Sample requirements

 

Urine

30 mL sterile universal container

(minimum of 5 mL urine is required)

 

or

 

 

transfer to Chlamydia urine pack

 

 

 

 

Swabs 

  • Genital-tract swab (cervical, male urethral or self-taken vaginal)
  • Pharyngeal swab
  • Rectal swab

Chlamydia swab pack

30mL sterile universal container

 Chlamydia urine pack

Chlamydia swab pack

Required information

  • Relevant clinical details

Storage/transport

Store urine at fridge temperature

Store transferred urines in Chlamydia transport medium at room temperature

Store swabs in Chlamydia transport medium at room temperature

Transport as soon as possible at ambient temperature

Turnaround time

Up to 4 days

All positive samples are confirmed locally which may slightly increase the turnaround time


Page last reviewed: 28/08/2014