Hepatitis B surface antibodies (Anti-HBs)




  • Used to determine immunity status after Hepatitis B vaccination
  • If Hepatitis B infection is suspected, please request Hepatitis B surface antigen
  • Samples giving a negative antibody result are tested for Hepatitis B core antibody to exclude natural hepatitis B infection as a cause of vaccine failure
  • Post vaccination testing is not routinely required except in certain high risk and occupational groups (see Immunisation Against Infectious Disease - The Green Book) Link to an external web site
  • Testing is not routinely required following a sharps/exposure incident

Sample requirements

Serum - paired samples not required

8.5ml of blood taken into a plain gel tube 

8.5mL BD Vacutainer tube

Required information

  • Relevant clinical details
  • Hepatitis B vaccination history


Store at fridge temperature

Transport as soon as possible at ambient temperature

Turnaround time

Up to 8 days

Page last reviewed: 18/11/2013