Hepatitis D virus (HDV)




  • Hepatitis D (Hepatitis Delta virus) is a blood borne virus
  • Hepatitis D virus can propagate only in people already infected with the Hepatitis B virus (HBV).
  • Super-infection and co-infection with HDV results in more severe complications compared to infection with HBV alone
  • Serology for Hepatitis D IgG and IgM is the screening test of choice.
  • Hepatitis D PCR is also available in relevant circumstances
  • See also Hepatitis B viral load

Sample requirements


Serum - paired samples not required

8.5 mL of blood taken into a plain gel tube



Plasma - paired samples not required

4ml of blood taken into an EDTA Tube

For adults 2 full EDTA tubes are required for Hepatitis D PCR


For babies and small children a minimum of one full 2mL paediatric EDTA or one full paediatric Mini Collect EDTA tube is required

8.5mL BD Vacutainer tube


4 mL EDTA tube



2 mL paediatric EDTA tube

     Paediatric Mini Collect EDTA tube

Required information

  • Relevant clinical details
  • Hepatitis B status
  • Time of sample collection



Store at fridge temperature

Transport as soon as possible at ambient temperature


Transport immediately at ambient temperature

Turnaround time

Hepatitis D serology and PCR:

Sent to a Regional Reference Centre

Up to 10 days



Page last reviewed: 15/05/2014