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Department of Haematology

Notes

  • Full blood counts are performed on automated equipment and provide haemoglobin concentration, red cell indices, white cell count (with a differential count) and platelet count.
  • The presence of abnormal white cell and red cell morphology is flagged by the analysers.
  • Blood films may be inspected to confirm and interpret abnormalities identified by the cell counter, or to look for certain specific haematological abnormalities.
  • Grossly abnormal FBC results and abnormal blood films will be phoned through to the requestor.
  • There is no need to request a blood film to obtain a differential white count. It is, however, important that clinical details are provided to allow the laboratory to decide whether a blood film, in addition to the automated analysis, is required.
  • Under some circumstances a differential is not routinely performed, e.g. pre-op, post-op, antenatal and postnatal requests.
  • Full Blood Counts are performed at CGH and GRH
  • See also: Reticulocyte Count

The FBC comprises the following tests

Standard

  • Haemoglobin (Hb)
  • White Blood Count (WBC)
  • Platelet Count (Plt)
  • Red Cell Count (RBC)
  • Haematocrit (HCT)
  • Mean Cell Volume - Red cell (MCV)
  • Mean Cell Haemoglobin (MCH)

Differential White Cell Count (where applicable)

  • Neutrophils
  • Lymphocytes
  • Monocytes
  • Eosinophils
  • Basophils

And if appropriate

  • Blood Film

Sample Requirements

2ml or 4ml EDTA sample or a Paediatric 1ml EDTA sample.

EDTA with cap

1ml Paediatric EDTA

Sample Storage and Retention

  • Pre analysis storage: do not store, send to laboratory within 4 hours.
  • Sample retention by lab: EDTA samples are retained for a minimum of 48 hours at 2-8°C
  • Transport of samples may affect sample viability, i.e. FBC results will degenerate if exposed to high temperatures, such as prolonged transportation in a hot car in summer.

This test can be added on to a previous request as long as there is sufficient sample remaining and the sample is less than 24 hours old.

Turnaround Times

  • Clinical emergency: 30 mins
  • Other urgent sample: 60 mins
  • Routine: within 2 hours

Reference Ranges

If references ranges are required for paediatric patients please contact the laboratory for these.

Parameter Patient Reference Range Units
Haemoglobin Adult Male 130 - 180 g/L
Adult Female 115 - 165 g/L
Red Cell Count Adult Male 4.50 - 6.50 x10^12/L
Adult Female 3.80 - 5.80 x10^12/L
Haematocrit Adult Male 0.40 - 0.54 L/L
Adult Female 0.37 - 0.47 L/L
Mean Cell Volume Adult 80 - 100 fL
Mean Cell Haemoglobin Adult 27 - 32 pg
White Cell Count Adult 3.6 - 11.0 x10^9/L
Neutrophils Adult 1.8 - 7.5 x10^9/L
Lymphocytes Adult 1.0 - 4.0 x10^9/L
Monocytes Adult 0.2 - 0.8 x10^9/L
Eosinophils Adult 0.1 - 0.4 x10^9/L
Basophils Adult 0.02 - 0.10 x10^9/L
Platelet Count Adult 140 - 400 x10^9/L