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Microbiology

Notes

Streptococcus

ASO antibodies

  • Streptolysin O is produced by almost all strains of S.pyogenes (group A streptococci) and many group C and group G beta haemolytic streptococci
  • May be used for diagnosis of recent streptococcal infections such as pharyngitis and tonsillitis but is unable to distinguish between infections with groups A, C and G streptococci. At best 80% of cases have positive ASO serology. The preferred method for acute sore throat is a bacterial throat swab for culture and sensitivity.
  • Useful for acute rheumatic fever, 80% positivity
  • Not very useful for infections such as impetigo and pyoderma
  • False positives may occur in jaundiced patients with liver disease
  • ASO rises 1 week after infection and peaks within 3-5 weeks returning to normal after 6-12 months

Sample requirements

Serum - paired samples not normally required

8.5ml of blood taken into a plain gel tube

Gold top with cap

Required information

  • Relevant clinical details
  • Date of onset

Storage/transport

Store at fridge temperature

Transport as soon as possible at ambient temperature

Turnaround time

Up to 8 days